The Role of Influenza A Virus Hemagglutinin Residues 226 and 228 in Receptor Specificity and Host Range Restriction
Identifieur interne : 001A40 ( Main/Exploration ); précédent : 001A39; suivant : 001A41The Role of Influenza A Virus Hemagglutinin Residues 226 and 228 in Receptor Specificity and Host Range Restriction
Auteurs : Angela Vines ; Krisna Wells ; Mikhail Matrosovich ; Maria R. Castrucci ; Toshihiro Ito ; Yoshihiro Kawaoka [États-Unis]Source :
- Journal of Virology [ 0022-538X ] ; 1998.
Abstract
Influenza A viruses can be isolated from a variety of animals, but their range of hosts is restricted. For example, human influenza viruses do not replicate in duck intestine, the major replication site of avian viruses in ducks. Although amino acids at positions 226 and 228 of hemagglutinin (HA) of the H3 subtype are known to be important for this host range restriction, the contributions of specific amino acids at these positions to restriction were not known. Here, we address this issue by generating HAs with site-specific mutations of a human virus that contain different amino acid residues at these positions. We also let ducks select replication-competent viruses from a replication-incompetent virus containing a human virus HA by inoculating animals with 1010.5 50% egg infectious dose of the latter virus and identified a mutation in the HA. Our results showed that the Ser-to-Gly mutation at position 228, in addition to the Leu-to-Gln mutation at position 226 of the HA of the H3 subtype, is critical for human virus HA to support virus replication in duck intestine.
Url:
PubMed: 9696865
PubMed Central: 110023
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000145
- to stream Pmc, to step Curation: 000145
- to stream Pmc, to step Checkpoint: 000C89
- to stream Ncbi, to step Merge: 001371
- to stream Ncbi, to step Curation: 001371
- to stream Ncbi, to step Checkpoint: 001371
- to stream Main, to step Merge: 001A92
- to stream Main, to step Curation: 001A40
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The Role of Influenza A Virus Hemagglutinin Residues 226 and 228 in Receptor Specificity and Host Range Restriction</title><author><name sortKey="Vines, Angela" sort="Vines, Angela" uniqKey="Vines A" first="Angela" last="Vines">Angela Vines</name><affiliation><nlm:aff id="N0x9c08f78.0x994a080"></nlm:aff></affiliation></author><author><name sortKey="Wells, Krisna" sort="Wells, Krisna" uniqKey="Wells K" first="Krisna" last="Wells">Krisna Wells</name><affiliation><nlm:aff id="N0x9c08f78.0x994a080"></nlm:aff></affiliation><affiliation><nlm:aff id="N0x9c08f78.0x994a080"></nlm:aff></affiliation></author><author><name sortKey="Matrosovich, Mikhail" sort="Matrosovich, Mikhail" uniqKey="Matrosovich M" first="Mikhail" last="Matrosovich">Mikhail Matrosovich</name><affiliation><nlm:aff id="N0x9c08f78.0x994a080"></nlm:aff></affiliation></author><author><name sortKey="Castrucci, Maria R" sort="Castrucci, Maria R" uniqKey="Castrucci M" first="Maria R." last="Castrucci">Maria R. Castrucci</name><affiliation><nlm:aff id="N0x9c08f78.0x994a080"></nlm:aff></affiliation></author><author><name sortKey="Ito, Toshihiro" sort="Ito, Toshihiro" uniqKey="Ito T" first="Toshihiro" last="Ito">Toshihiro Ito</name><affiliation><nlm:aff id="N0x9c08f78.0x994a080"></nlm:aff></affiliation></author><author><name sortKey="Kawaoka, Yoshihiro" sort="Kawaoka, Yoshihiro" uniqKey="Kawaoka Y" first="Yoshihiro" last="Kawaoka">Yoshihiro Kawaoka</name><affiliation><nlm:aff id="N0x9c08f78.0x994a080"></nlm:aff><country>États-Unis</country><placeName><settlement type="city">Madison (Wisconsin)</settlement><region type="state">Wisconsin</region></placeName><orgName type="university" n="3">Université du Wisconsin à Madison</orgName></affiliation><affiliation><nlm:aff id="N0x9c08f78.0x994a080"></nlm:aff><country>États-Unis</country><placeName><settlement type="city">Madison (Wisconsin)</settlement><region type="state">Wisconsin</region></placeName><orgName type="university" n="3">Université du Wisconsin à Madison</orgName></affiliation><affiliation><nlm:aff id="N0x9c08f78.0x994a080"></nlm:aff><country>États-Unis</country><placeName><settlement type="city">Madison (Wisconsin)</settlement><region type="state">Wisconsin</region></placeName><orgName type="university" n="3">Université du Wisconsin à Madison</orgName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">9696865</idno><idno type="pmc">110023</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC110023</idno><idno type="RBID">PMC:110023</idno><date when="1998">1998</date><idno type="wicri:Area/Pmc/Corpus">000145</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000145</idno><idno type="wicri:Area/Pmc/Curation">000145</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000145</idno><idno type="wicri:Area/Pmc/Checkpoint">000C89</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000C89</idno><idno type="wicri:Area/Ncbi/Merge">001371</idno><idno type="wicri:Area/Ncbi/Curation">001371</idno><idno type="wicri:Area/Ncbi/Checkpoint">001371</idno><idno type="wicri:doubleKey">0022-538X:1998:Vines A:the:role:of</idno><idno type="wicri:Area/Main/Merge">001A92</idno><idno type="wicri:Area/Main/Curation">001A40</idno><idno type="wicri:Area/Main/Exploration">001A40</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The Role of Influenza A Virus Hemagglutinin Residues 226 and 228 in Receptor Specificity and Host Range Restriction</title><author><name sortKey="Vines, Angela" sort="Vines, Angela" uniqKey="Vines A" first="Angela" last="Vines">Angela Vines</name><affiliation><nlm:aff id="N0x9c08f78.0x994a080"></nlm:aff></affiliation></author><author><name sortKey="Wells, Krisna" sort="Wells, Krisna" uniqKey="Wells K" first="Krisna" last="Wells">Krisna Wells</name><affiliation><nlm:aff id="N0x9c08f78.0x994a080"></nlm:aff></affiliation><affiliation><nlm:aff id="N0x9c08f78.0x994a080"></nlm:aff></affiliation></author><author><name sortKey="Matrosovich, Mikhail" sort="Matrosovich, Mikhail" uniqKey="Matrosovich M" first="Mikhail" last="Matrosovich">Mikhail Matrosovich</name><affiliation><nlm:aff id="N0x9c08f78.0x994a080"></nlm:aff></affiliation></author><author><name sortKey="Castrucci, Maria R" sort="Castrucci, Maria R" uniqKey="Castrucci M" first="Maria R." last="Castrucci">Maria R. Castrucci</name><affiliation><nlm:aff id="N0x9c08f78.0x994a080"></nlm:aff></affiliation></author><author><name sortKey="Ito, Toshihiro" sort="Ito, Toshihiro" uniqKey="Ito T" first="Toshihiro" last="Ito">Toshihiro Ito</name><affiliation><nlm:aff id="N0x9c08f78.0x994a080"></nlm:aff></affiliation></author><author><name sortKey="Kawaoka, Yoshihiro" sort="Kawaoka, Yoshihiro" uniqKey="Kawaoka Y" first="Yoshihiro" last="Kawaoka">Yoshihiro Kawaoka</name><affiliation><nlm:aff id="N0x9c08f78.0x994a080"></nlm:aff><country>États-Unis</country><placeName><settlement type="city">Madison (Wisconsin)</settlement><region type="state">Wisconsin</region></placeName><orgName type="university" n="3">Université du Wisconsin à Madison</orgName></affiliation><affiliation><nlm:aff id="N0x9c08f78.0x994a080"></nlm:aff><country>États-Unis</country><placeName><settlement type="city">Madison (Wisconsin)</settlement><region type="state">Wisconsin</region></placeName><orgName type="university" n="3">Université du Wisconsin à Madison</orgName></affiliation><affiliation><nlm:aff id="N0x9c08f78.0x994a080"></nlm:aff><country>États-Unis</country><placeName><settlement type="city">Madison (Wisconsin)</settlement><region type="state">Wisconsin</region></placeName><orgName type="university" n="3">Université du Wisconsin à Madison</orgName></affiliation></author></analytic><series><title level="j">Journal of Virology</title><idno type="ISSN">0022-538X</idno><idno type="eISSN">1098-5514</idno><imprint><date when="1998">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Influenza A viruses can be isolated from a variety of animals, but their range of hosts is restricted. For example, human influenza viruses do not replicate in duck intestine, the major replication site of avian viruses in ducks. Although amino acids at positions 226 and 228 of hemagglutinin (HA) of the H3 subtype are known to be important for this host range restriction, the contributions of specific amino acids at these positions to restriction were not known. Here, we address this issue by generating HAs with site-specific mutations of a human virus that contain different amino acid residues at these positions. We also let ducks select replication-competent viruses from a replication-incompetent virus containing a human virus HA by inoculating animals with 10<sup>10.5</sup> 50% egg infectious dose of the latter virus and identified a mutation in the HA. Our results showed that the Ser-to-Gly mutation at position 228, in addition to the Leu-to-Gln mutation at position 226 of the HA of the H3 subtype, is critical for human virus HA to support virus replication in duck intestine.</p></div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Wisconsin</li></region><settlement><li>Madison (Wisconsin)</li></settlement><orgName><li>Université du Wisconsin à Madison</li></orgName></list><tree><noCountry><name sortKey="Castrucci, Maria R" sort="Castrucci, Maria R" uniqKey="Castrucci M" first="Maria R." last="Castrucci">Maria R. Castrucci</name><name sortKey="Ito, Toshihiro" sort="Ito, Toshihiro" uniqKey="Ito T" first="Toshihiro" last="Ito">Toshihiro Ito</name><name sortKey="Matrosovich, Mikhail" sort="Matrosovich, Mikhail" uniqKey="Matrosovich M" first="Mikhail" last="Matrosovich">Mikhail Matrosovich</name><name sortKey="Vines, Angela" sort="Vines, Angela" uniqKey="Vines A" first="Angela" last="Vines">Angela Vines</name><name sortKey="Wells, Krisna" sort="Wells, Krisna" uniqKey="Wells K" first="Krisna" last="Wells">Krisna Wells</name></noCountry><country name="États-Unis"><region name="Wisconsin"><name sortKey="Kawaoka, Yoshihiro" sort="Kawaoka, Yoshihiro" uniqKey="Kawaoka Y" first="Yoshihiro" last="Kawaoka">Yoshihiro Kawaoka</name></region><name sortKey="Kawaoka, Yoshihiro" sort="Kawaoka, Yoshihiro" uniqKey="Kawaoka Y" first="Yoshihiro" last="Kawaoka">Yoshihiro Kawaoka</name><name sortKey="Kawaoka, Yoshihiro" sort="Kawaoka, Yoshihiro" uniqKey="Kawaoka Y" first="Yoshihiro" last="Kawaoka">Yoshihiro Kawaoka</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001A40 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001A40 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= H2N2V1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:110023
|texte= The Role of Influenza A Virus Hemagglutinin Residues 226 and 228 in Receptor Specificity and Host Range Restriction
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:9696865" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a H2N2V1
| This area was generated with Dilib version V0.6.33. |  |